Complement Decay-Accelerating Factor (CD55) antibody

Antigen: Complement Decay-Accelerating Factor (CD55)

Synonyms: CR, TC, DAF, CROM

Clonality: Monoclonal (MEM-118)

Host: Mouse

Reactivity: Human, Primates

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94183

Additional Information

Alternative name: CD55

Immunogen: HPB-ALL human T cell line

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: MEM-118

Description: CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein thatprotects autologous cells from classical and alternative pathway of complement cascade.Bidirectional interactions between CD55 and CD97 are involved in T cell regulation andCD55 can still regulate complement when bound to CD97. In tumours, besides protectionagains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness andevasion of apoptosis.

Specificity: The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decayaccelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored singlechain glycoprotein. CD55 is widely expressed on hematopoietic and on manynon-hematopoietic cells, it is weakly present on NK cells.HLDA V, WS Code AS S016

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and size-exclusion chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Research Area: Immunology, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Angelisovua, Drbal, Horejsui et al.: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).