CD55 antibody (Complement Decay-Accelerating Factor) (Domain SCR4) (Biotin)

Details for Product anti-CD55 Antibody No. ABIN94184, Supplier: Log in to see
Antigen
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CR
  • CROM
  • DAF
  • TC
  • AI323748
  • Cd55b
  • Daf-TM
  • TM-DAF
  • CD55 molecule (Cromer blood group)
  • CD55 molecule, decay accelerating factor for complement
  • CD55 molecule, decay accelerating factor for complement B
  • Cd55
  • CD55
  • Cd55b
Alternatives
anti-Human CD55 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Domain SCR4
18
12
10
9
9
8
8
8
7
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Non-Human Primate
415
56
22
9
9
3
3
2
2
2
Host
Mouse
338
100
24
8
7
3
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD55 antibody is conjugated to Biotin
53
47
29
15
13
11
11
10
8
8
6
6
6
6
6
5
5
5
4
4
4
3
3
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
359
188
97
65
58
54
41
39
12
8
6
6
6
5
3
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen HPB-ALL human T cell line
Clone MEM-118
Isotype IgM
Specificity The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.
HLDA V, WS Code AS S016
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System, Regulation of Leukocyte Mediated Immunity
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-Complement Decay-Accelerating Factor (CD55) (Domain SCR4) antibody (Biotin) (ABIN94184) Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 a...
Background publications VanLandingham, Cekic, Cutler, Hoffman, Stein: "Neurosteroids reduce inflammation after TBI through CD55 induction." in: Neuroscience letters, Vol. 425, Issue 2, pp. 94-8, 2007 (PubMed).

Abbott, Spendlove, Roversi, Fitzgibbon, Knott, Teriete, McDonnell, Handford, Lea: "Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 22023-32, 2007 (PubMed).

Miwa, Maldonado, Zhou, Yamada, Gilkeson, Eisenberg, Song: "Decay-accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement-dependent and -independent mechanisms." in: The American journal of pathology, Vol. 170, Issue 4, pp. 1258-66, 2007 (PubMed).

Mikesch, Buerger, Simon, Brandt: "Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies." in: Biochimica et biophysica acta, Vol. 1766, Issue 1, pp. 42-52, 2006 (PubMed).

Miwa, Maldonado, Zhou, Sun, Luo, Cai, Werth, Madaio, Eisenberg, Song: "Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice." in: The American journal of pathology, Vol. 161, Issue 3, pp. 1077-86, 2002 (PubMed).

Product cited in: Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).

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