CD95 antibody
| Antigen |
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| Clonality |
Monoclonal (UT-1) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Functional Studies (Func)
Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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| Catalog no. |
ABIN94250 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD95 / Fas
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Immunogen
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HUT-78 human T cell lymphoma cells
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Format
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Azide Free
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Isotype
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IgM (Matching secondary antibodies)
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Clone
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UT-1
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Description
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CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of theTNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular deathdomains, formation of the death-inducing signaling complex (DISC) and activation ofcaspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated atthe DISC, in type II cells low concentration of caspase 8 activates pathway leading to therelease of cytochrome c from mitochondria and activation of caspase 3 by cytochom c.Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokineresponses.
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Specificity
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The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumournecrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a varietyof normal and neoplastic cells.The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11antibody.
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Application Notes
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Functional Application: The antibody UT-1 induces Fas-mediated apoptosis. Flow Cytometry
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by precipitation methods and ion exchange chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 ยต m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD95", type "Antibodies"
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Hosts
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Mouse (91), Rabbit (20), Goat (1)
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Reactivities
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Human (109), Monkey (3), Mouse (Murine) (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (63), Western Blotting (WB) (34), Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Functional Studies (Func) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Blocking Antibody (Inhibition) (8), ELISA (6), Immunofluorescence (IF) (5), ELISA (Detection) (3), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2)
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Conjugates
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FITC (10), PE (10), APC (6), Biotin (4), SPRD (2)
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Epitopes
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C-Term (4), pTyr232 (2), pTyr291 (2), Center (1)
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