Clusterin antibody

Catalog No. ABIN94264

Product Details anti-Clusterin Antibody

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J), a conserved secreted glycoprotein.

No Cross-Reactivity: Cat, Cow, Dog, Pig

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Clone: Hs-3

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: Sertoli cells.
Western blotting: Recommended dilution: 1 μg/mL, positive material: seminal plasma.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Clusterin antibody (ABIN94264)

Capková, Geussová, Peknicová: "New monoclonal antibody to human apolipoprotein J." in: Folia biologica, Vol. 48, Issue 1, pp. 40-2, (2002) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin (CLU Products)

Synonyms: LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody

Background: Clusterin,Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.,Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, CLU, NA1/NA2, Apolipoprotein J, Apo-J, TRPM-2, KBP1, KUB1, CLI, AAG4, APOJ, SGP2

Gene ID: 1191

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling