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PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term)

Details for Product anti-PAG1 Antibody No. ABIN94270, Supplier: Log in to see
Antigen
  • MGC80192
  • CBP
  • PAG
  • Cbp
  • F730007C19Rik
  • Pag
  • 8430414N03Rik
  • IGFBP-4ase
  • PAG1
  • PAPP-A
Alternatives
anti-Human PAG1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
21
14
3
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
54
27
20
3
Host
Mouse
36
22
Clonality (Clone)
Monoclonal ()
Conjugate
This PAG1 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
35
25
19
11
7
7
3
1
Supplier
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Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name PAG1 (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, EGFR Signaling Pathway
Application Notes Immunoprecipitation:
Positive control: RAJI human Burkitt lymphoma cell line

Immunohistochemistry (paraffin sections)
Positive tissue: Appendix (germinal center of lymphatic follicle
Application note: heat-mediated antigen retrieval in citrate buffer pH 6.1

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunoprecipitation (IP) image for anti-PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term) (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Immunohistochemistry (IHC) image for anti-PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term) (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...
Product cited in: Vang, Abrahamsen, Myklebust et al.: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).

Davidson, Bakinowski, Thomas et al.: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).

Background publications Simeoni, Smida, Posevitz et al.: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004 (PubMed).

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004 (PubMed).

Baumgartner, Angelisová, Setterblad et al.: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 (PubMed).