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phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody

Details for Product No. ABIN94270
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Antigen
Synonyms MGC80192, CBP, PAG, Cbp, F730007C19Rik, Pag, 8430414N03Rik, IGFBP-4ase, PAG1, PAPP-A
Epitope
C-Term
(18), (8), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
(47), (18), (16), (3)
Host
Mouse
(26), (24)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(29), (20), (19), (10), (9), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN94270
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Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name PAG1
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Application Notes Immunoprecipitation:
Positive control: RAJI human Burkitt lymphoma cell line

Immunohistochemistry (paraffin sections)
Positive tissue: Appendix (germinal center of lymphatic follicle
Application note: heat-mediated antigen retrieval in citrate buffer pH 6.1

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1). Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (2) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells
General Baumgartner, Angelisová, Setterblad et al.: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 (PubMed).

Davidson, Bakinowski, Thomas et al.: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).

Vang, Abrahamsen, Myklebust et al.: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).

Validation Images
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