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Antibody
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anti-Cytokeratin 10 KRT10
Cytokeratin 10 (KRT10) antibody
| Antigen |
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| Synonyms |
K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik |
| Clonality |
Monoclonal (VIK-10) |
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Host
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Reactivity
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Application
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 Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
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| Catalog no. |
ABIN94277 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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Cytokeratin 10
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Immunogen
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Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction.
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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VIK-10
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Description
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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
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Specificity
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The antibody VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues.
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Application Notes
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Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: skinPretreatment: trypsin/temperature 37 o C
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 10 (KRT10)", type "Antibodies"
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Hosts
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Mouse (25), Rabbit (11), Guinea Pig (1)
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Reactivities
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Human (35), Rat (Rattus) (5), Dog (Canine) (4), Mouse (Murine) (4), Cat (Feline) (3), Cow (Bovine) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Western Blotting (WB) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Immunofluorescence (IF) (10), ELISA (4), Enzyme Immunoassay (EIA) (4), Flow Cytometry (FACS) (4), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (2)
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Conjugates
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FITC (1)
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Epitopes
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N-Term (3), Center (1)
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