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FYN antibody (AA 7-176)

FYN Reactivity: Human, Mouse WB, IHC (p), IP, ICC Host: Mouse Monoclonal FYN-01 unconjugated
Catalog No. ABIN94311
  • Target See all FYN Antibodies
    FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
    Binding Specificity
    • 13
    • 12
    • 9
    • 8
    • 8
    • 7
    • 7
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 7-176
    Reactivity
    • 93
    • 64
    • 37
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 91
    • 14
    • 1
    Mouse
    Clonality
    • 90
    • 16
    Monoclonal
    Conjugate
    • 61
    • 9
    • 8
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This FYN antibody is un-conjugated
    Application
    • 86
    • 66
    • 26
    • 9
    • 8
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
    Cross-Reactivity (Details)
    Mouse, Human, Other not tested
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
    Clone
    FYN-01
    Isotype
    IgG2b
  • Application Notes
    Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: brain.
    Western blotting: Recommended dilution: 2 μg/mL, positive control: JURKAT human T cell leukemia cell line.
    Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, (2011) (PubMed).

    Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

  • Target
    FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
    Alternative Name
    Fyn (FYN Products)
    Synonyms
    im:6908851 antibody, si:dkey-119m7.5 antibody, zgc:109996 antibody, c-fyn antibody, cfyn-a antibody, SLK antibody, SYN antibody, p59-FYN antibody, PKC antibody, fyn antibody, zgc:86720 antibody, AI448320 antibody, AW552119 antibody, FYN proto-oncogene, Src family tyrosine kinase antibody, FYN proto-oncogene, Src family tyrosine kinase S homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase b antibody, FYN proto-oncogene, Src family tyrosine kinase L homeolog antibody, FYN proto-oncogene, Src family tyrosine kinase a antibody, Fyn proto-oncogene antibody, FYN antibody, fyn.S antibody, fynb antibody, fyn.L antibody, Fyn antibody, fyna antibody
    Background
    FYN proto-oncogene, Src family tyrosine kinase,Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.,p59Fyn, SLK, SYN
    Gene ID
    2534
    UniProt
    P06241
    Pathways
    JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
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