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Tubulin, gamma 1 (TUBG1) (C-Term), (AA 434-449) antibody

Details for Product No. ABIN94313
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Antigen
Synonyms TUBC, TUBG, TUBG3, gamma-tubulin, GCP-1, TUBGCP1, 1500010O08Rik, AI451582, AI503389, Tubg, tubgl, zgc:55894, Tubg2, xgam
Epitope
C-Term, AA 434-449
(12), (10), (9), (9), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
(53), (13), (10), (9), (8), (7), (3), (3), (2), (2), (1), (1), (1), (1)
Host
Mouse
(33), (28)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Western Blotting (WB)
(51), (17), (16), (12), (11), (6), (5), (2), (1)
Pubmed 11 references available
Quantity 0.1 mg
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Catalog No. ABIN94313
348.48 $
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  • +1 888 205 9894 (TF)
Immunogen C-terminal peptide of gamma-tubulin counjugated to KLH.
Clone TU-30
Isotype IgG1
Specificity The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name gamma-tubulin
Background The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Gene ID 17q21
Application Notes Immunocytochemistry: Recommended dilution: Purified Antibody: 1-2 µg/mL
Staining technique: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C.
Incubation: 45 min in room temperature
Positive control: P-19 mouse embryonal carcinoma cell line3T3 mouse fibroblasts
Application note: The antibody TU-30 stains only fixed cells.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-Tubulin, gamma 1 (TUBG1) (C-Term), (AA 434-449) antibody Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue). Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
anti-Tubulin, gamma 1 (TUBG1) (C-Term), (AA 434-449) antibody (2) anti-Tubulin, gamma 1 (TUBG1) (C-Term), (AA 434-449) antibody (Image 2)
Product cited in: Löffler, Fechter, Matuszewska et al.: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, 2011 (PubMed).

Vulprecht, David, Tibelius et al.: "STIL is required for centriole duplication in human cells." in: Journal of cell science, 2012 (PubMed).

General Nováková, Dráberová, Schürmann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Dráberová, Dráberová, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).

Binarová, Cenklová, Hause et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Libusová, Sulimenko, Sulimenko et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Mi, Gan, Cheema et al.: "Cancer preventive isothiocyanates induce selective degradation of cellular {alpha}- and {beta}-tubulins by proteasomes." in: The Journal of biological chemistry, Vol. 284, Issue 25, pp. 17039-51, 2009 (PubMed).

Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Tibelius, Marhold, Zentgraf et al.: "Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1." in: The Journal of cell biology, Vol. 185, Issue 7, pp. 1149-57, 2009 (PubMed).

Mi, Gan, Chung: "Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 456-62, 2009 (PubMed).

Koledova, Kafkova, Kraemer et al.: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, 2010 (PubMed).

Validation Images
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