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TUBG1 antibody (Tubulin, gamma 1) (C-Term)

Details for Product anti-TUBG1 Antibody No. ABIN94313, Supplier: Log in to see
Antigen
  • TUBC
  • TUBG
  • TUBG3
  • gamma-tubulin
  • GCP-1
  • TUBGCP1
  • 1500010O08Rik
  • AI451582
  • AI503389
  • Tubg
  • tubgl
  • zgc:55894
  • Tubg2
  • xgam
  • gamma-tubulin1
  • tubulin gamma-1 chain
  • tubulin gamma-1 chain-like
  • tubulin, gamma 1
  • tubg1
  • TUBG1
  • Tubg1
Alternatives
anti-Human TUBG1 antibody for Immunocytochemistry
Epitope
C-Term, AA 434-449
19
12
10
8
6
2
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
64
29
22
9
8
8
7
6
5
5
4
3
2
2
2
2
1
1
1
Host
Mouse
44
25
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBG1 antibody is un-conjugated
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
63
18
13
11
10
9
7
3
1
1
Options
Supplier
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Immunogen C-terminal peptide of gamma-tubulin counjugated to KLH.
Clone TU-30
Isotype IgG1
Specificity The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name gamma-tubulin (TUBG1 Antibody Abstract)
Background The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Pathways Microtubule Dynamics, M Phase
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence stai...
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse ...
Immunofluorescence (IF) image for anti-TUBG1 antibody (Tubulin, gamma 1) (C-Term) (ABIN94313) Fig. A, B, C Immunofluorescence staining (mouse fibroblasts) Immunofluorescence stain...
Product cited in: Vulprecht, David, Tibelius, Castiel, Konotop, Liu, Bestvater, Raab, Zentgraf, Izraeli, Krämer: "STIL is required for centriole duplication in human cells." in: Journal of cell science, 2012 (PubMed).

Löffler, Fechter, Matuszewska, Saffrich, Mistrik, Marhold, Hornung, Westermann, Bartek, Krämer: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, 2011 (PubMed).

Koledova, Kafkova, Kraemer, Divoky: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, 2010 (PubMed).

Katsetos, Reddy, Dráberová, Smejkalová, Del Valle, Ashraf, Tadevosyan, Yelin, Maraziotis, Mishra, Mörk, Legido, Nissanov, Baas, de Chadarévian, Dráber: "Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines." in: Journal of neuropathology and experimental neurology, Vol. 65, Issue 5, pp. 465-77, 2006 (PubMed).

Sulimenko, Dráberová, Sulimenko, Macurek, Richterová, Dráber, Dráber: "Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7243-53, 2006 (PubMed).

Libusová, Sulimenko, Sulimenko, Hozák, Dráber: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Binarová, Cenklová, Hause, Kubátová, Lysák, Dolezel, Bögre, Dráber: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Background publications Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Tibelius, Marhold, Zentgraf, Heilig, Neitzel, Ducommun, Rauch, Ho, Bartek, Kraemer: "Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1." in: The Journal of cell biology, Vol. 185, Issue 7, pp. 1149-57, 2009 (PubMed).

Mi, Gan, Cheema, Dakshanamurthy, Wang, Yang, Chung: "Cancer preventive isothiocyanates induce selective degradation of cellular {alpha}- and {beta}-tubulins by proteasomes." in: The Journal of biological chemistry, Vol. 284, Issue 25, pp. 17039-51, 2009 (PubMed).

Mi, Gan, Chung: "Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 456-62, 2009 (PubMed).

Wiese, Zheng: "Microtubule nucleation: gamma-tubulin and beyond." in: Journal of cell science, Vol. 119, Issue Pt 20, pp. 4143-53, 2006 (PubMed).

Dráberová, Dráberová, Surviladze, Dráber, Dráber: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).