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Details for Product No. ABIN94313

gamma-Tubulin (TUBG) (C-Term) antibody

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Antigen
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Western Blotting (WB)
Pubmed 11 references available
Catalog no. ABIN94313
Quantity 0.1 mg
Price
348.48 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen C-terminal peptide of gamma-tubulin counjugated to KLH.
Clone TU-30
Isotype IgG1
Specificity The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubuleorganizing center (MTOC).
Purification Purified from rabbit serum by protein-A afinity chromatography.
Purity > 95% (by SDS-PAGE)
Alternative Name gamma-tubulin
Background The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.
Gene ID 17q21
Application Notes Immunocytochemistry - Recommended dilution: Purified Antibody: 1-2 µg/ml Staining technique: (a) Fix cells for 10 min in methanol at -20 o C and for 6 min in acetone at -20 o C, (b) Fix cells directly in methanol for 10 min at -20 o C or in acetone for 10 min at -20 o C. Incubation: 45 min in room temperaturePositive control: P-19 mouse embryonal carcinoma cell line3T3 mouse fibroblasts. Application note: The antibody TU-30 stains only fixed cells.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C
Storage Comment Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Löffler, Fechter, Matuszewska et al.: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, 2011 (PubMed).

Vulprecht, David, Tibelius et al.: "STIL is required for centriole duplication in human cells." in: Journal of cell science, 2012 (PubMed).

Nováková, Dráberová, Schürmann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Dráberová, Dráberová, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).

Binarová, Cenklová, Hause et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Libusová, Sulimenko, Sulimenko et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Mi, Gan, Cheema et al.: "Cancer preventive isothiocyanates induce selective degradation of cellular {alpha}- and {beta}-tubulins by proteasomes." in: The Journal of biological chemistry, Vol. 284, Issue 25, pp. 17039-51, 2009 (PubMed).

Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Tibelius, Marhold, Zentgraf et al.: "Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1." in: The Journal of cell biology, Vol. 185, Issue 7, pp. 1149-57, 2009 (PubMed).

Mi, Gan, Chung: "Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 456-62, 2009 (PubMed).

Koledova, Kafkova, Kraemer et al.: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, 2010 (PubMed).

Alternatives for antigen "gamma-Tubulin (TUBG)", type "Antibodies"
Hosts (4)
Reactivities (4), (4), (1), (1), (1), (1), (1), (1)
Applications (2), (2), (1)
Conjugates (1), (1), (1)
Epitopes (1)
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