Tubulin, gamma 1 (TUBG1) antibody (Dyomics 547)

Details for Product No. ABIN94314
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Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
(48), (10), (7), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1)
(31), (23)
Clonality (Clone)
Monoclonal ()
Dyomics 547
(1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC)
(48), (15), (11), (10), (9), (6), (5), (5), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN94314
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Immunogen C-terminal peptide of gamma-tubulin counjugated to KLH.
Clone TU-30
Isotype IgG1
Specificity The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubuleorganizing center (MTOC).
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Alternative Name TUBG1
Background The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.
Application Notes Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1: 500.It is recommended that the user titrates the reagent for use in the particular testing system.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Tubulin, gamma 1 (TUBG1) antibody (Dyomics 547) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue). Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
anti-Tubulin, gamma 1 (TUBG1) antibody (Dyomics 547) (2) anti-Tubulin, gamma 1 (TUBG1) antibody (Dyomics 547) (Image 2)
General Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Libusová, Sulimenko, Sulimenko et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Experimental cell research, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Binarová, Cenklová, Hause et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: The Plant cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Dráberová, Dráberová, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).

Nováková, Dráberová, Schürmann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

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