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Tubulin gamma 1 (TUBG1) antibody (Dyomics 547)
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant
Alternatives Dyomics 547
|5 references available|
|Quantity||0.1 mg (1 mg/ml)|
|Price||471.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Immunogen||C-terminal peptide of gamma-tubulin counjugated to KLH.|
|Description||The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.|
|Specificity||The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubuleorganizing center (MTOC).|
|Synonyms||TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827|
|Application Notes||Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1: 500.It is recommended that the user titrates the reagent for use in the particular testing system.|
|Purification||Purified from hybridoma culture supernatant by protein-A affinity chromatography.|
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage||Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.|
|Restrictions||For Research Use only|
Novuakovua, Druaberovua, Schuermann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).
Draberova, Draberova, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).
Binarova P, Cenklova V, Hause B et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: Plant Cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).
Libusova L, Sulimenko T, Sulimenko V et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Exp Cell Res, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).
Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).
|Hosts||Mouse (9), Rabbit (2)|
|Reactivities||Human (9), Plant (9), Rat (Rattus) (9), Mouse (Murine) (8), Chicken (7), Cow (Bovine) (7), Pig (Porcine) (7), Protozoa (3), All Species (1)|
|Applications||Western Blotting (WB) (10), Immunocytochemistry (ICC) (8), Immunohistochemistry (IHC) (2), ELISA (1)|
|Conjugates||DyLight 549 (2), Dyomics 647 (2)|
|Epitopes||C-Term (5), AA 434-449 (1)|