Tubulin gamma 1 (TUBG1) antibody (Dyomics 647)

Antigen: Tubulin gamma 1 (TUBG1)

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Clonality: Monoclonal (TU-30)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant

Conjugate: Dyomics 647

Catalog no.: ABIN94315

Additional Information

Alternative name: gamma-tubulin

Gene ID: 17q21

Immunogen: C-terminal peptide of gamma-tubulin counjugated to KLH.

Isotype: IgG1

Clone: TU-30

Description: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.

Specificity: The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubuleorganizing center (MTOC).

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Application Details

Application Notes: Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1: 100.The conjugate was also successfully used on paraffin sections using confocal microscopy. It is recommended that the user titrates the reagent for use in the particular testing system.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue). Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).

anti-Tubulin gamma 1 (TUBG1) antibody (Dyomics 647) (Image 2)

Publications

Publications: Novuakovua, Druaberovua, Schuermann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Draberova, Draberova, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).

Binarova P, Cenklova V, Hause B et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: Plant Cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Libusova L, Sulimenko T, Sulimenko V et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Exp Cell Res, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).