HLA Class I antibody

Antigen: HLA Class I

Clonality: Monoclonal (W6-32)

Host: Mouse

Reactivity: Human, Primates, Cow (Bovine), Cat (Feline)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN94332

Additional Information

Alternative name: HLA-Class I

Immunogen: Membrane of human tonsil cells

Format: Azide Free

Isotype: IgG2a  (Matching secondary antibodies)

Clone: W6-32

Description: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules.

Specificity: The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types.The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expressionin different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathyand other neuromuscular disorders.This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. This antibody does not react with: Rabbit.

Application Details

Application Notes: Western Blotting. Application note: Non-reducing conditions. Functional Application: The antibody W6/32 is suitable as a positive control for HLA tissue typing.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein G-affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) Alexa Fluor® 488. 

Publications

Publications: Gonzalez, Alegre, Torres et al.: "Evaluation of HLA-G5 Plasmatic Levels During Pregnancy and Relationship with the 14-bp Polymorphism." in: American journal of reproductive immunology (New York, N.Y. : 1989), 2010 (PubMed).