HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (87G)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), ELISA

Catalog no.: ABIN94362

Additional Information

Alternative name: HLA-G

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.

Format: Azide Free

Isotype: IgG2a

Clone: 87G

Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat.

Application Details

Application Notes: Flow Cytometry - Positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections) - Recommended dilution: 10 µg/ml Incubation: 20 min at 25 o C Positive tissue: extravillous cytotrophoblastELISARecommended dilution: 1 µg/ml. Positive control: JEG-3 human choriocarcinoma epithelial cell line. Application note: The antibody 87G has been tested as the capture antibody in a sandwichELISA for analysis of human HLA-G in combination with antibody W6/32. Functional Application. Application note: The antibody 87G blocks interaction of HLA-G with inhibitory receptors.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Analysis of cytolytical activity

Publications

Publications: Riteau, Menier, Khalil-Daher et al.: "HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition." in: International immunology, Vol. 13, Issue 2, pp. 193-201, 2001 (PubMed).

Wiendl, Mitsdoerffer, Hofmeister et al.: "The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy." in: Brain : a journal of neurology, Vol. 126, Issue Pt 1, pp. 176-85, 2002 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Poluakovua, Krcovua, Kuba et al.: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Sageshima, Ishitani, Omura et al.: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 49, Issue 3, pp. 174-82, 2003 (PubMed).

Ishitani, Sageshima, Lee et al.: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

Poluakovua, Bandzuchovua, Hofmeister et al.: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Hackmon, Hallak, Krup et al.: "HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy." in: Fetal diagnosis and therapy, Vol. 19, Issue 5, pp. 404-9, 2004 (PubMed).

Rouas-Freiss, Moreau, Ferrone et al.: "HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism?" in: Cancer research, Vol. 65, Issue 22, pp. 10139-44, 2005 (PubMed).

Shobu, Sageshima, Tokui et al.: "The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation." in: Journal of reproductive immunology, Vol. 72, Issue 1-2, pp. 18-32, 2006 (PubMed).

LeMaoult, Caumartin, Daouya et al.: "Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells." in: Blood, Vol. 109, Issue 5, pp. 2040-8, 2007 (PubMed).

Valencic, Piscianz, Andolina et al.: "The immunosuppressive effect of Wharton's jelly stromal cells depends on the timing of their licensing and on lymphocyte activation." in: Cytotherapy, Vol. 12, Issue 2, pp. 154-60, 2010 (PubMed).

Alegre, Howangyin, Favier et al.: "Membrane redistributions through multi-intercellular exchanges and serial trogocytosis." in: Cell research, Vol. 20, Issue 11, pp. 1239-51, 2010 (PubMed).

Giuliani, Fleury, Vernochet et al.: "Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation." in: PLoS ONE, Vol. 6, Issue 5, pp. e19988, 2011 (PubMed).

Ketroussi, Giuliani, Bahri et al.: "Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway." in: PLoS ONE, Vol. 6, Issue 8, pp. e22776, 2011 (PubMed).