HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

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Antigen
Reactivity
Human
(316), (8), (4), (1)
Host
Mouse
(199), (100), (20), (6), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(40), (35), (29), (19), (9), (5), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), ELISA
(172), (160), (134), (58), (52), (50), (41), (30), (28), (24), (8), (7), (3), (2), (1), (1), (1), (1), (1)
Pubmed 15 references available
Quantity 0.1 mg
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Immunogen HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.
Clone 87G
Isotype IgG2a
Specificity The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name HLAG (HLAG Antibody Abstract)
Research Area Major Histocompatibility Complex (MHC)
Application Notes Flow Cytometry - Positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections) - Recommended dilution: 10 µg/ml Incubation: 20 min at 25 o C Positive tissue: extravillous cytotrophoblastELISARecommended dilution: 1 µg/ml. Positive control: JEG-3 human choriocarcinoma epithelial cell line. Application note: The antibody 87G has been tested as the capture antibody in a sandwichELISA for analysis of human HLA-G in combination with antibody W6/32. Functional Application. Application note: The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
Preservative Azide free
Storage 4 °C
Supplier Images
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody Analysis of cytolytical activity
Product cited in: Ketroussi, Giuliani, Bahri et al.: "Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway." in: PLoS ONE, Vol. 6, Issue 8, pp. e22776, 2011 (PubMed).

Giuliani, Fleury, Vernochet et al.: "Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation." in: PLoS ONE, Vol. 6, Issue 5, pp. e19988, 2011 (PubMed).

Alegre, Howangyin, Favier et al.: "Membrane redistributions through multi-intercellular exchanges and serial trogocytosis." in: Cell research, Vol. 20, Issue 11, pp. 1239-51, 2010 (PubMed).

General Valencic, Piscianz, Andolina et al.: "The immunosuppressive effect of Wharton's jelly stromal cells depends on the timing of their licensing and on lymphocyte activation." in: Cytotherapy, Vol. 12, Issue 2, pp. 154-60, 2010 (PubMed).

LeMaoult, Caumartin, Daouya et al.: "Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells." in: Blood, Vol. 109, Issue 5, pp. 2040-8, 2007 (PubMed).

Shobu, Sageshima, Tokui et al.: "The surface expression of HLA-F on decidual trophoblasts increases from mid to term gestation." in: Journal of reproductive immunology, Vol. 72, Issue 1-2, pp. 18-32, 2006 (PubMed).

Rouas-Freiss, Moreau, Ferrone et al.: "HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism?" in: Cancer research, Vol. 65, Issue 22, pp. 10139-44, 2005 (PubMed).

Hackmon, Hallak, Krup et al.: "HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy." in: Fetal diagnosis and therapy, Vol. 19, Issue 5, pp. 404-9, 2004 (PubMed).

Poláková, Bandzuchová, Hofmeister et al.: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Ishitani, Sageshima, Lee et al.: "Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1376-84, 2003 (PubMed).

Sageshima, Ishitani, Omura et al.: "Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 49, Issue 3, pp. 174-82, 2003 (PubMed).

Poláková, Krcová, Kuba et al.: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Wiendl, Mitsdoerffer, Hofmeister et al.: "The non-classical MHC molecule HLA-G protects human muscle cells from immune-mediated lysis: implications for myoblast transplantation and gene therapy." in: Brain : a journal of neurology, Vol. 126, Issue Pt 1, pp. 176-85, 2002 (PubMed).

Riteau, Menier, Khalil-Daher et al.: "HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition." in: International immunology, Vol. 13, Issue 2, pp. 193-201, 2001 (PubMed).

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Catalog No. ABIN94362
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