HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (87G)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS), ELISA

Catalog no.: ABIN94363

Additional Information

Alternative name: HLA-G

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.

Isotype: IgG2a

Clone: 87G

Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule onthe cell surface, and for ELISA. Suggested working dilution for Flow Cytometry is 1: 500.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Thereagent is free of unconjugated biotin..

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Analysis of cytolytical activity