HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (87G)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94364

Additional Information

Alternative name: HLA-G

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.

Isotype: IgG2a

Clone: 87G

Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1: 100.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimumconditions. The reagent is free of unconjugated FITC..

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Analysis of cytolytical activity