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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN94369, Supplier: Log in to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
  • major histocompatibility complex, class I, G
  • MHC BF1 class I
  • HLA-G
  • BF1
Reactivity
Human
508
8
7
4
1
Host
Mouse
411
73
26
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
50
36
28
12
9
9
9
9
9
9
9
9
9
9
8
8
8
7
5
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
296
283
276
155
137
131
130
110
74
22
21
8
7
3
3
2
1
1
1
1
Options
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Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Clone MEM-G-9
Isotype IgG1
Specificity The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
No Cross-Reactivity Mouse (Murine)
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Alternative Name HLAG (HLAG Antibody Abstract)
Research Area Major Histocompatibility Complex (MHC)
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN94369) Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human ...
Immunofluorescence (IF) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN94369) Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA...
Immunoprecipitation (IP) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN94369) Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA...
Product cited in: Zhao, Teklemariam, Hantash: "Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies." in: Tissue antigens, Vol. 80, Issue 3, pp. 231-8, 2012 (PubMed).

Gauster, Berghold, Moser, Orendi, Siwetz, Huppertz: "Fibulin-5 expression in the human placenta." in: Histochemistry and cell biology, Vol. 135, Issue 2, pp. 203-13, 2011 (PubMed).

Giuliani, Fleury, Vernochet, Ketroussi, Clay, Azzarone, Lataillade, Durrbach: "Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation." in: PLoS ONE, Vol. 6, Issue 5, pp. e19988, 2011 (PubMed).

Gonzalez, Alegre, Arroyo, LeMaoult, Echeveste: "Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates." in: Clinical chemistry, Vol. 57, Issue 7, pp. 1013-22, 2011 (PubMed).

Ongaro, Stignani, Pellati, Melchiorri, Massari, Caruso, De Mattei, Caruso, Baricordi, Rizzo: "Human leukocyte antigen-G molecules are constitutively expressed by synovial fibroblasts and upmodulated in osteoarthritis." in: Human immunology, Vol. 71, Issue 4, pp. 342-50, 2010 (PubMed).

Shaikly, Shakhawat, Withey, Morrison, Taranissi, Dealtry, Jabeen, Cherry, Fernuandez: "Cell bio-imaging reveals co-expression of HLA-G and HLA-E in human preimplantation embryos." in: Reproductive biomedicine online, Vol. 20, Issue 2, pp. 223-233, 2010 (PubMed).

Morandi, Ferretti, Bocca, Prigione, Raffaghello, Pistoia: "A novel mechanism of soluble HLA-G mediated immune modulation: downregulation of T cell chemokine receptor expression and impairment of chemotaxis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11763, 2010 (PubMed).

Alegre, Howangyin, Favier, Baudhuin, Lesport, Daouya, Gonzalez, Carosella, Lemaoult: "Membrane redistributions through multi-intercellular exchanges and serial trogocytosis." in: Cell research, Vol. 20, Issue 11, pp. 1239-51, 2010 (PubMed).

López, Alegre, LeMaoult, Carosella, González: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." in: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, 2006 (PubMed).

Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Fuzzi, Rizzo, Criscuoli, Noci, Melchiorri, Scarselli, Bencini, Menicucci, Baricordi: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." in: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, 2002 (PubMed).

Lozano, González, Kindelán, Rouas-Freiss, Caballos, Dausset, Carosella, Peña: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." in: AIDS, Vol. 16, Issue 3, pp. 347-51, 2002 (PubMed).

Pangault, Le Friec, Caulet-Maugendre, Léna, Amiot, Guilloux, Onno, Fauchet: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." in: Human immunology, Vol. 63, Issue 2, pp. 83-90, 2002 (PubMed).

Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).