Mouse anti-Human IgE Antibody

Catalog No. ABIN94398

Product Details

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).

Cross-Reactivity (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE.

Clone: 4G7-325

Isotype: IgG

Application Details

Application Notes: Flow cytrometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Smith, Benjamin, Hozic, Derewenda, Smith, Thomas, Gafvelin, van Hage-Hamsten, Chapman: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).

Mueller, Smith, Chapman, Rule, Benjamin: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).

Target Details

Target: IgE

Abstract: IgE Products

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molecular Weight: 180 kDa