Linker For Activation of T Cells (LAT) antibody

Antigen: Linker For Activation of T Cells (LAT)

Synonyms: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Clonality: Monoclonal (LAT-1D1)

Host: Mouse

Reactivity: Mouse (Murine), Rat (Rattus)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94420

Additional Information

Alternative name: LAT

Immunogen: Bacterially expressed full-length rat LAT

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: LAT-1D1

Description: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembraneadaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Afterimmunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, orTec-family kinases, providing docking sites for downstream signaling molecules. LAT isessential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as wellas for maturation of early thymocytes. It is also involved in NK cell signaling and plateletactivation.

Specificity: The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), atransmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells andplatelets. This antibody does not react with: Human.

Application Details

Application Notes: Western Blotting - Recommended dilution: 5-10 µg/ml. Positive control: mouse spleen

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Adaptive Immunity

Restrictions: For Research Use only

Images

Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC, 1A) and RBL rat basophilic leukemia cell line (1B) by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody (cat. no. 11-487-C100), followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.

Publications

Publications: Tolar P, Tumova M, Draber P: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia Biol (Praha), Vol. 47, Issue 6, pp. 215-7, 2007 (PubMed).