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Phosphotyrosine antibody

Name: Phosphotyrosine antibody
Brand: antibodies-online
SKU: ABIN94452
Price: 384.05 USD
Availability: InStock

Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated

Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/mL, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel).

FACS

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor ® 488 (green).

2 images

(3 references)

Catalog No. ABIN94452

Datasheet as PDF

Target See all Phosphotyrosine products

Phosphotyrosine
Reactivity
19

16

16

4

1
Various Species
Host
89

28

1
Mouse
Compatible Secondaries
Clonality
101

17
Monoclonal
Conjugate
41

16

10

8

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Phosphotyrosine antibody is un-conjugated
Application
84

61

40

38

37

27

22

17

13

9

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity

The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Cross-Reactivity (Details)

Broad

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Phosphotyrosine conjugated to bovine serum albumin.

Clone

P-Tyr-01

Isotype

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, FACS, ELISA, ICC Host: Mouse Monoclonal E10 unconjugated

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal FITC

anti-Phosphotyrosine antibody (Atto 488)
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal Atto 488

anti-Phosphotyrosine antibody (Atto 594)
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal Atto 594

anti-Phosphotyrosine antibody (Atto 390)
WB, IP, IF, IHC, ELISA, ICC Host: Rabbit Polyclonal Atto 390

anti-Phosphotyrosine antibody
WB, IP, IHC, FACS, ELISA, ICC Host: Rabbit Monoclonal RM111 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated

Application Notes

Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Recommended dilution: 2-5 μg/mL. Intracellular staining.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Biebl, Riedl, Buchner: "Hsp90 Co-chaperones Form Plastic Genetic Networks Adapted to Client Maturation." in: Cell reports, Vol. 32, Issue 8, pp. 108063, (2020) (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
Target

Phosphotyrosine

Abstract

Phosphotyrosine Products

Target Type

Amino Acid

Background

PY