STAT1 antibody

Catalog No. ABIN94471

Product Details anti-STAT1 Antibody

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Cross-Reactivity (Details): Mouse, Human, Other not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).

Clone: SM1

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: lung macrophages.
Western blotting: Recommended dilution 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-STAT1 antibody (ABIN94471)

Kovarik, Boudny, Kocak, Lauerova, Fait, Vagundova: "Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation." in: International journal of molecular medicine, Vol. 12, Issue 3, pp. 335-40, (2003) (PubMed).

Boudný, Kocák, Lauerová, Kovarík: "Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients." in: Folia biologica, Vol. 49, Issue 4, pp. 142-6, (2003) (PubMed).

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1 (STAT1 Products)

Synonyms: CANDF7 antibody, ISGF-3 antibody, STAT91 antibody, 2010005J02Rik antibody, AA408197 antibody, XStat1 antibody, STAT4 antibody, DD6G4-4 antibody, STAT antibody, STAT1 antibody, dZ182H3.3 antibody, si:by134g18.2 antibody, si:by51f19.1 antibody, si:dz182h3.3 antibody, si:dz199m19.1 antibody, si:dz73o4.2 antibody, stat1 antibody, signal transducer and activator of transcription 1 antibody, signal transducer and activator of transcription 1 L homeolog antibody, signal transducer and activator of transcription 1, 91kDa antibody, signal transducer and activator of transcription 4 antibody, signal transducer and activator of transcription 1a antibody, STAT1 antibody, Stat1 antibody, stat1.L antibody, stat4 antibody, stat1a antibody

Background: Signal transducer and activator of transcription 1,STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.,CANDF7, IMD31, ISGF3

Gene ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events