Actin antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Clonality: Monoclonal (HHF35)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94597

Additional Information

Immunogen: BALB/C mice were injected with SDS extracted protein from human myocardium.

Format: Supernatant

Isotype: IgG1-kappa  (Matching secondary antibodies)

Clone: HHF35

Specificity: This antibody is specific to alpha and gamma specific actin isomers from skeletal, cardiac and smooth muscle but does not recognize beta and nonsmooth muscle gamma actin isomers. In Western blot, HHF35 picks up 42 kD protein extract from skeletal muscle but not from epithelial cell line.

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon stained with Muscle Actin antibody

Publications

Publications: Porter, Bigler, McNutt et al.: "The immunophenotype of hemangiopericytomas and glomus tumors, with special reference to muscle protein expression: an immunohistochemical study and review of the literature." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 4, Issue 1, pp. 46-52, 1991 (PubMed).

Gown, Tsukada, Ross: "Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions." in: The American journal of pathology, Vol. 125, Issue 1, pp. 191-207, 1986 (PubMed).