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Actin antibody
| Antigen | Actin |
| Synonyms |
A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G ...
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| Clonality | Monoclonal (5C5) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Cow (Bovine), Rabbit, Guinea Pig, Rat (Rattus), Frog, Snake, Carp |
| Application |
Alternatives Immunohistochemistry (IHC)
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3 references available |
| Catalog no. | ABIN94603 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 362.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business days |
Additional Information
| Immunogen | BALB/C mice were injected with purified rabbit striated muscle actin. |
| Format | Ig-fraction |
| Isotype | IgM (Matching secondary antibodies) |
| Clone | 5C5 |
| Specificity | Monoclonal anti-alpha-sarcomeric Actin has been used as a marker for rhabdomyosarcoma. This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It cross reacts with human, sheep, bovine, rabbit, guinea pig, rat, frog, snake and carp. It does not reacts with smooth muscle tissue. |
| Synonyms | A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051 |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as diluted ascites containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Images
| Formalin fixed paraffin embedded human skeletal muscle stained with Actin, sarcomeric antibody |
Publications
| Publications |
Schuerch, Skalli, Seemayer et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors." in: The American journal of pathology, Vol. 128, Issue 1, pp. 91-103, 1987 (PubMed).
Babai, Skalli, Schurch et al.: "Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 55, Issue 5, pp. 263-77, 1988 (PubMed). Skalli, Gabbiani, Babaui et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas." in: The American journal of pathology, Vol. 130, Issue 3, pp. 515-31, 1988 (PubMed). |
Alternatives
Alternatives for antigen "Actin", type "Antibodies"
| Hosts | Mouse (58), Rabbit (18), Sheep (3) |
| Reactivities | Human (46), Chicken (23), Rat (Rattus) (19), Mouse (Murine) (12), Cow (Bovine) (10), Rabbit (9), Snake (7), Carp (5), Frog (5), Guinea Pig (5), Sheep (Ovine) (5), All Species (3), Dog (Canine) (3), Cat (Feline) (2), Pig (Porcine) (2), Xenopus laevis (2), Dictyostelium (1), Horse (Equine) (1), Physarum (1), Primate (1), Primates (1) |
| Applications | Western Blotting (WB) (54), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), Immunofluorescence (IF) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), ELISA (16), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (7), Enzyme Immunoassay (EIA) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (6), Cy3 (1), FITC (1) |
| Epitopes | C-Term (6), pan (6), N-Term (3), Ab-55/53 (1), Asp244 (1), pTyr55/53 (1) |
