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Actinin antibody
| Antigen |
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| Clonality |
Monoclonal (EA-53) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN94616 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
331.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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Purified rabbit skeletal alpha-actinin.
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Format
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Ascites
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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EA-53
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Specificity
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This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non- sarcomeric muscle elements. This antibody shows wide range of cross reactivity with various mammalian species.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Skeletal muscle. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as diluted ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Goncharova, Kam, Geiger: "The involvement of adherens junction components in myofibrillogenesis in cultured cardiac myocytes." in: Development (Cambridge, England), Vol. 114, Issue 1, pp. 173-83, 1992 (PubMed).
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Alternatives for antigen "Actinin", type "Antibodies"
Alternatives 
