Adenovirus antibody

Antigen: Adenovirus

Clonality: Monoclonal (M58-M73)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94714

Additional Information

Immunogen: BALB/C mice were injected with adenovirus.

Format: Ig-fraction

Isotype: IgG2a-kappa  (Matching secondary antibodies)

Clone: M58-M73

Specificity: This antibody reacts with adenovirus. The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Adenovirus infected tissue. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human infected appendix stained with Adenovirus antibody

Publications

Publications: Harlow, Whyte, Franza et al.: "Association of adenovirus early-region 1A proteins with cellular polypeptides." in: Molecular and cellular biology, Vol. 6, Issue 5, pp. 1579-89, 1987 (PubMed).

Klein: "The approaching era of the tumor suppressor genes." in: Science (New York, N.Y.), Vol. 238, Issue 4833, pp. 1539-45, 1988 (PubMed).

Harlow, Franza, Schley: "Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products." in: Journal of virology, Vol. 55, Issue 3, pp. 533-46, 1985 (PubMed).