Aldolase (ALD) antibody

Antigen: Aldolase (ALD)

Synonyms: ALD, BcDNA:LP07735, FBA, T12, T4, T6, T7, DmelCG6058, CG6058

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN94900

Additional Information

Alternative name: Aldolase

Immunogen: Aldolase [Rabbit Muscle]Aldolase [Rabbit Muscle] Antibody

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code ABIN101212 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate. Optimal titers for other applications should be determined by the researcher. suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Concentration: 90.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Cancer, Metabolism, Enzymes

Restrictions: For Research Use only

Images

Immunoblotting. IgG purified antibody to rabbit muscle aldolase (ABIN94900, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4