ATP5J antibody (Middle Region)

Catalog No. ABIN950574

Product Details anti-ATP5J Antibody

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Binding Specificity: AA 27-56, Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to ATPase subunit F6.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 27-56 amino acids from the Central region of human ATP5J

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: ATPase Subunit F6 (ATP5J Products)

Synonyms: zgc:77541 antibody, wu:fa66e04 antibody, ATP5 antibody, ATP5A antibody, ATPM antibody, CF6 antibody, F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F antibody, atp5j antibody, ATP5J antibody, atp5j.L antibody, Atp5j antibody

Background: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.Synonyms: ATP synthase, ATP synthase-coupling factor 6 mitochondrial, ATP5A, ATP5J, ATPM, Complex V subunit F6, F1F0 ATPase subunit F6, H+ transporting, mitochondrial F0 complex, subunit F6

Gene ID: 522

NCBI Accession: NP_001003696

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process