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BTF3 antibody (Basic Transcription Factor 3) (N-Term)

Details for Product anti-BTF3 Antibody No. ABIN950687, Supplier: Log in to see
Antigen
  • BTF3
  • nacb
  • btf3a
  • btf3b
  • beta-nac
  • ci-btf3
  • ATBTF3
  • F2H15.11
  • F2H15_11
  • basic transcription factor 3
  • wu:fb05d06
  • zgc:153435
  • BETA-NAC
  • BTF3a
  • BTF3b
  • NACB
  • 1700054E11Rik
Epitope
N-Term, AA 1-30
17
16
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
73
21
17
3
2
2
2
1
Host
Rabbit
61
10
2
1
Clonality
Polyclonal
Conjugate
This BTF3 antibody is un-conjugated
6
6
6
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
56
36
11
10
4
4
3
3
3
2
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BTF3
Isotype Ig
Specificity This antibody reacts to BTF3.
Cross-Reactivity (Details) Species reactivity (tested):Human and Mouse.
Purification Affinity chromatography on Protein A
Alternative Name BTF3 (BTF3 Antibody Abstract)
Background This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.Synonyms: NACB, OK/SW-cl.8, RNA polymerase B transcription factor 3, Transcription factor BTF3
Gene ID 689
NCBI Accession NP_001032726
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-BTF3 antibody (Basic Transcription Factor 3) (N-Term) (ABIN950687) BTF3 Antibody (N-term) western blot analysis in A549 cell line lysates (35µg/lane).Th...
Western Blotting (WB) image for anti-BTF3 antibody (Basic Transcription Factor 3) (N-Term) (ABIN950687) BTF3 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35µg/la...
Background publications Green, Thompson, Johnston et al.: "Interaction between transcription factor, basal transcription factor 3, and the NH2-terminal domain of human estrogen receptor alpha." in: Molecular cancer research : MCR, Vol. 5, Issue 11, pp. 1191-200, 2007 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Kusumawidjaja, Kayed, Giese et al.: "Basic transcription factor 3 (BTF3) regulates transcription of tumor-associated genes in pancreatic cancer cells." in: Cancer biology & therapy, Vol. 6, Issue 3, pp. 367-76, 2007 (PubMed).

Freire: "Translation initiation factor (iso) 4E interacts with BTF3, the beta subunit of the nascent polypeptide-associated complex." in: Gene, Vol. 345, Issue 2, pp. 271-7, 2005 (PubMed).

Harrington, Sherf, Rundlett et al.: "Creation of genome-wide protein expression libraries using random activation of gene expression...." in: Nature biotechnology, Vol. 19, Issue 5, pp. 440-5, 2001 (PubMed).