GNAT3 antibody (Middle Region)

Catalog No. ABIN952565

Product Details anti-GNAT3 Antibody

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Binding Specificity: AA 85-113, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAT3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human GNAT3 (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of Human GNAT3

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GNAT3

Target: GNAT3 (Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3))

Alternative Name: GNAT3 (GNAT3 Products)

Synonyms: GDCA antibody, Ggust antibody, Gtn antibody, gnat-3 antibody, G protein subunit alpha transducin 3 antibody, guanine nucleotide binding protein, alpha transducing 3 antibody, GNAT3 antibody, Gnat3 antibody

Background: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase, Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.Synonyms: G protein G(t) subunit alpha-3, Guanine nucleotide-binding protein G(t) subunit alpha-3, Gustducin alpha-3 chain

Molecular Weight: 40357 Da

Gene ID: 346562

NCBI Accession: NP_001095856

Pathways: Peptide Hormone Metabolism, G-protein mediated Events, Phototransduction