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Details for Product No. ABIN953252

Ly6A/E / SCA1 antibody (PE)

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Antigen
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 5 references available
Catalog no. ABIN953252
Quantity 50 µg
Price
324.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Clone CT-6A-6E
Isotype IgG2b
Specificity This monoclonal antibody recognizes Sca-1 (Ly-6A.2/6E.1), a cell surface antigen used in the identification of hematopoietic stem cells.
Purification Affinity chromatography on Protein G
Background Ly6A/E is a member of the Ly-6 antigen family. The Thy-1lo, Lin- (lineage-negative, not expressing B220, Gr-1, Mac-1, CD4 or CD8), Sca-1+ population of bone marrow cells are highly purified, perhaps homogenous, pluripotent stem cells. This antigen is also present on various other tissues. Specific staining of the parenchymal cells can be demonstrated in thymus, spleen and kidney where as only vasculature reacts with anti-Sca-1 in brain, heart and liver (and possibly in lung). Also, Sca-1 is a T cell activation antigen, as surface expression of the antigen increases upon Con A activation of T lymphocytes. Sca-1 appears to have a molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions, indicating the presence of intra-chain disulfide bonds.
Alternate names: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP
Gene ID 110454
NCBI Accession NP_034868
UniProt P05533
Restrictions For Research Use only
Format Liquid
Concentration 0,1 mg/mL
Buffer PBS containing 0.02% Sodium azide as preservativeAhighly purified grade of BSA has been added as a stablizing protein to bring the final protein concentration to 4-5 mg/ml after conjugation.
Preservative Sodium azide
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Codias, Cray, Baler et al.: "Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes." in: Immunogenetics, Vol. 29, Issue 2, pp. 98-107, 1989 (PubMed).

van de Rijn, Heimfeld, Spangrude et al.: "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 12, pp. 4634-8, 1989 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

Tomonari: "A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex." in: Immunogenetics, Vol. 28, Issue 6, pp. 455-8, 1988 (PubMed).

Aihara, Bühring, Aihara et al.: "An attempt to produce \pre-T\" cell hybridomas and to identify their antigens."" in: European journal of immunology, Vol. 16, Issue 11, pp. 1391-9, 1987 (PubMed).

Alternatives for antigen "Ly6A/E / SCA1", type "Antibodies"
Hosts (13), (1)
Reactivities (14)
Applications (13), (3), (3), (1), (1), (1)
Conjugates (3), (2), (1), (1), (1), (1)
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