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MED16 antibody (Mediator Complex Subunit 16) (AA 252-281)

Details for Product anti-MED16 Antibody No. ABIN953369, Supplier: Log in to see
Antigen
  • THRAP5
  • MED16
  • DRIP92
  • TRAP95
  • 95kDa
  • A630083L04
  • Thrap5
  • Trap95
Epitope
AA 252-281, Middle Region
15
13
11
6
5
4
3
2
2
1
1
1
Reactivity
Human
40
5
4
3
1
Host
Rabbit
32
8
Clonality
Polyclonal
Conjugate
This MED16 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
38
22
5
3
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human MED16
Isotype Ig
Specificity This antibody recognizes Human MED16 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED16 (MED16 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Synonyms: DRIP92, Mediator complex subunit 16, Mediator of RNA polymerase II transcription subunit 16, THRAP5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP92
Molecular Weight 72kd
Gene ID 10025
NCBI Accession NP_005472
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-MED16 antibody (Mediator Complex Subunit 16) (AA 252-281) (ABIN953369) Western blot analysis of MED16 Antibody (Center) in 293 cell line lysates (35ug/lane)...
Background publications Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Wang, Sharma, Ren et al.: "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42852-8, 2002 (PubMed).

Kang, Guermah, Yuan et al.: "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2642-7, 2002 (PubMed).

Suzuki, Tsunoda, Sese et al.: "Identification and characterization of the potential promoter regions of 1031 kinds of human genes." in: Genome research, Vol. 11, Issue 5, pp. 677-84, 2001 (PubMed).