MED16 antibody (Mediator Complex Subunit 16) (AA 252-281)

Details for Product anti-MED16 Antibody No. ABIN953369, Supplier: Log in to see
Antigen
  • THRAP5
  • MED16
  • DRIP92
  • TRAP95
  • 95kDa
  • A630083L04
  • Thrap5
  • Trap95
  • mediator complex subunit 16
  • MED16
  • Med16
  • med16
Alternatives
anti-Human MED16 antibody for ELISA
Epitope
AA 252-281, Middle Region
14
11
9
7
6
5
4
2
2
2
1
1
1
1
1
1
Reactivity
Human
51
10
8
7
6
6
4
4
2
2
2
1
Host
Rabbit
36
15
Clonality
Polyclonal
Conjugate
This MED16 antibody is un-conjugated
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
48
29
8
8
3
2
2
Options
Supplier
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Immunogen KLH conjugated synthetic peptide between 252-281 amino acids from the Central region of human MED16
Isotype Ig
Specificity This antibody recognizes Human MED16 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED16 (MED16 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Synonyms: DRIP92, Mediator complex subunit 16, Mediator of RNA polymerase II transcription subunit 16, THRAP5, Thyroid hormone receptor-associated protein complex 95 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP92
Molecular Weight 72kd
Gene ID 10025
NCBI Accession NP_005472
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-MED16 antibody (Mediator Complex Subunit 16) (AA 252-281) (ABIN953369) Western blot analysis of MED16 Antibody (Center) in 293 cell line lysates (35ug/lane)...
Background publications Sato, Tomomori-Sato, Parmely, Florens, Zybailov, Swanson, Banks, Jin, Cai, Washburn, Conaway, Conaway: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely, Banks, Sorokina, Florens, Zybailov, Washburn, Brower, Conaway, Conaway: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Wang, Sharma, Ren, Fondell: "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42852-8, 2002 (PubMed).

Kang, Guermah, Yuan, Roeder: "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 2642-7, 2002 (PubMed).

Suzuki, Tsunoda, Sese, Taira, Mizushima-Sugano, Hata, Ota, Isogai, Tanaka, Nakamura, Suyama, Sakaki, Morishita, Okubo, Sugano: "Identification and characterization of the potential promoter regions of 1031 kinds of human genes." in: Genome research, Vol. 11, Issue 5, pp. 677-84, 2001 (PubMed).