MED8 antibody (Mediator Complex Subunit 8) (AA 102-130)

Details for Product anti-MED8 Antibody No. ABIN953379, Supplier: Log in to see
Antigen
  • zgc:76877
  • med8-a
  • ARC32
  • 2210021A15Rik
  • AB041805
  • MNCb-2386
  • mediator complex subunit 8
  • component of RNA polymerase II
  • mediator of RNA polymerase II transcription, subunit 8 homolog (yeast)
  • med8
  • MED8
  • Med8
Alternatives
anti-Human MED8 antibody for ELISA
Epitope
AA 102-130, Middle Region
10
10
10
8
5
3
3
3
2
1
1
1
Reactivity
Human
39
10
7
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
32
7
Clonality
Polyclonal
Conjugate
This MED8 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
38
25
5
3
3
2
Options
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Immunogen KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human MED8
Isotype Ig
Specificity This antibody recognizes Human MED8 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED8 (MED8 Antibody Abstract)
Background This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.Synonyms: Activator-recruited cofactor 32 kDa component, Mediator complex 8, Mediator of RNA polymerase II transcription subunit 8
Molecular Weight 29080 Da
Gene ID 112950
NCBI Accession NP_443109
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-MED8 antibody (Mediator Complex Subunit 8) (AA 102-130) (ABIN953379) Western blot analysis of MED8 Antibody (Center) in HL-60 cell line lysates (35ug/lane...
Background publications Léonard, Kohl, Hart, Elliott: "Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response." in: Journal of virology, Vol. 80, Issue 19, pp. 9667-75, 2006 (PubMed).

Wang, Yoon, Oh, Jeon, Kim, Kim, Byun, Yang, Kim, Kim, Yeom, Yoo, Kim, Kim: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006 (PubMed).

Sato, Tomomori-Sato, Parmely, Florens, Zybailov, Swanson, Banks, Jin, Cai, Washburn, Conaway, Conaway: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely, Banks, Sorokina, Florens, Zybailov, Washburn, Brower, Conaway, Conaway: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Sato, Tomomori-Sato, Banks, Parmely, Sorokina, Brower, Conaway, Conaway: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).