MGMT antibody (N-Term)

Catalog No. ABIN953415

Product Details anti-MGMT Antibody

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: Synthetic peptide (between 1-30 aa) - KLH conjugated - corresponding to the N-terminal region of human MGMT.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT (MGMT Products)

Synonyms: AGT antibody, AI267024 antibody, Agat antibody, O-6-methylguanine-DNA methyltransferase antibody, MGMT antibody, Mgmt antibody

Background: MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. It repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.Synonyms: 6-O-methylguanine-DNA methyltransferase, Methylated-DNA-protein-cysteine methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene ID: 9606

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus