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Nitrilase 1 (NIT1) (AA 297-327), (C-Term) antibody

Details for Product No. ABIN953707, Supplier: Log in to see
Antigen
  • A. THALIANA NITRILASE 1
  • AI255805
  • ATNIT1
  • DDBDRAFT_0217173
  • DDBDRAFT_0302491
  • DDB_0217173
  • DDB_0302491
  • ESTM30
  • MGC85476
  • NITI
  • nitrilase 1
  • NITRILE AMINOHYDROLASE
  • W57327
  • zgc:101630
  • ZmNIT1
Epitope
C-Term, AA 297-327
17
15
6
2
1
1
1
Reactivity
Human
53
18
18
Host
Rabbit
44
9
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
40
22
11
9
8
6
5
2
Supplier
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Immunogen Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 297-327aa) of human NIT1
Isotype Ig
Specificity This antibody recognizes human NIT1 at C-term.
Purification Purified through a Protein A column followed by peptide affinity purification
Alternative Name NIT1 (NIT1 Antibody Abstract)
Background The NIT1 gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: Nitrilase homolog 1
Molecular Weight 35896 Da
Gene ID 4817
NCBI Accession NP_001172021
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Nitrilase 1 (NIT1) (AA 297-327), (C-Term) antibody (ABIN953707) Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) using NIT1  Antibody  ...
Background publications Semba, Han, Qin, McCorkell, Iliopoulos, Pekarsky, Druck, Trapasso, Croce, Huebner: "Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 28244-53, 2006

Wang, Yoon, Oh, Jeon, Kim, Kim, Byun, Yang, Kim, Kim, Yeom, Yoo, Kim, Kim: "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1022-32, 2006

Pekarsky, Campiglio, Siprashvili, Druck, Sedkov, Tillib, Draganescu, Wermuth, Rothman, Huebner, Buchberg, Mazo, Brenner, Croce: "Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8744-9, 1998