Aspartate, L- antibody

Antigen: Aspartate, L-

Clonality: Polyclonal

Host: Rabbit

Application: Immunocytochemistry (ICC)

Catalog no.: ABIN95403

Additional Information

Immunogen: Synthetic L-aspartic acid conjugated to protein carrier (Pc)

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Specificity: Conjugate L-aspartic acid . Using a conjugate L-aspartic acid-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) L-Aspartic acid-G-(Pc) 1 L-Glutamic-G-(Pc) 1/30,000 GABA-G-(Pc) 1/>100,000 (a): L-Aspartic-G-(Pc) concentration / other conjugated aminoacids concentration at half displacement G = Glutaraldehyde

Application Details

Application Notes: Recommended dilutions for Immunocytochemistry (1/1,000-5,000) Recommended dilutions for Western Blot (1/1,000-1/2,000)

Purity: Antiserum previously preabsobed on protein carriers, and purified

Storage: Lyophilized antibodies are stable at least 2 years. After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times).

Research Area: Amino Acids

Restrictions: For Research Use only

Publications

Publications: Campistron, Buijs, Geffard: "Specific antibodies against aspartate and their immunocytochemical application in the rat brain." in: Brain research, Vol. 365, Issue 1, pp. 179-84, 1986 (PubMed).

Sinakevitch, Farris, Strausfeld: "Taurine-, aspartate- and glutamate-like immunoreactivity identifies chemically distinct subdivisions of Kenyon cells in the cockroach mushroom body." in: The Journal of comparative neurology, Vol. 439, Issue 3, pp. 352-67, 2001 (PubMed).

Sjoeholm, Sinakevitch, Strausfeld et al.: "Functional division of intrinsic neurons in the mushroom bodies of male Spodoptera littoralis revealed by antibodies against aspartate, taurine, FMRF-amide, Mas-allatotropin and DC0." in: Arthropod structure & development, Vol. 35, Issue 3, pp. 153-68, 2007 (PubMed).