PF4 antibody

Catalog No. ABIN954175

Product Details anti-PF4 Antibody

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Human Platelet Factor IV. AM31850PU-N (clone RTO) is seen to bind free PF4 in the absence of any bound Heparin. This is unlike AM31851PU-N (clone KKO), which binds PF4/heparin complexes to a similar degree as auto-antibodies found in individuals with Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography of Ascitic fluid.

Immunogen: Human Factor IV purified from the supernatant of Thrombin activated platelets.

Clone: RTO

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4 / PF4 (PF4 Products)

Synonyms: CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody

Background: Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gene ID: 5196

NCBI Accession: NP_002610

UniProt: P02776