Ataxia Telangiectasia Mutated (ATM) antibody

Antigen: Ataxia Telangiectasia Mutated (ATM)

Synonyms: AT1, ATA, ATC, ATD, ATE, ATDC, TEL1, TELO1, MGC74674, DKFZp781A0353, AI256621, C030026E19Rik, ATM, Tefu, atm/tefu, dATM, tef, DmelCG6535, CG6535, Xatm

Clonality: Monoclonal (ATX08)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN95420

Additional Information

Immunogen: BALB/C mice were injected with recombinant human ATM protein.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: ATX08

Specificity: This antibody reacts with a 350 kD protein known as ATM (Ataxia Telangiectasia Mutated). ATM protein phosphorylates p53 on serine 15 near the N terminus, thereby contributing to the activation and stabilization of p53 during the IR-induced DNA damage response. This antibody cross reacts with mouse.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with ATM antibody

Publications

Publications: Crawford: "Ataxia telangiectasia." in: Seminars in pediatric neurology, Vol. 5, Issue 4, pp. 287-94, 1999 (PubMed).

Knittweis: "An ataxia telangiectasia model: inefficient cell differentiation and possible reversal by serine protease inhibitors, tumor necrosis factor inhibitors, dexamethasone, and glutathione enhancers." in: Medical hypotheses, Vol. 51, Issue 1, pp. 53-7, 1999 (PubMed).