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anti-ATM Protein Kinase S1981 antibody

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Antigen: ATM Protein Kinase S1981
Clonality: Monoclonal (10H11.E12)
Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)
Reactivity: Human, Mouse (Murine)
Host: Mouse
8 references available 8 references available
Quantity: 100ug (1.0 mg/ml (by UV absorbance at...)
Price: 355,00 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN95421
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Additional Information  » anti-ATM Protein Kinase S1981 antibody

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Antigen ATM Protein Kinase S1981
Immunogen This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Reactivity Human, Mouse (Murine)
Clonality Monoclonal
Format Liquid (sterile filtered)
Isotype IgG1 (kappa) »Matching secondary antibodies
Description ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G 1 , S and G 2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G 1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G 2 /M checkpoint.
Clone 10H11.E12
Host Mouse
Specificity This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.

Application Details  » anti-ATM Protein Kinase S1981 antibody

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Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
» Click here to view 3 available protocols!
Application Notes Protein A Purified Mab anti-ATM has been tested by ELISA and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and mouse cells. By immunofluoresence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8. Recommended Dilutions: ELISA 1:20,000 - 1:100,000. WESTERN BLOT 1:200 - 1:2,000. IF MICROSCOPY 1:100 - 1:500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Storage Store vial at -20° C. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Research Area Kinases/Phosphatases, Cancer, Neurology, DNA/RNA
Restrictions For Research Use only

Publications  » anti-ATM Protein Kinase S1981 antibody

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Publications Powers, Hong, Mayhew et al.: "E2F1 uses the ATM signaling pathway to induce p53 and Chk2 phosphorylation and apoptosis." In: Molecular cancer research : MCR , Vol. 2, Issue 4, pp. 203-14, 2004 (PubMed)

Pusapati, Rounbehler, Hong et al.: "ATM promotes apoptosis and suppresses tumorigenesis in response to Myc." In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 103, Issue 5, pp. 1446-51, 2006 (PubMed)

Biton, Gropp, Itsykson et al.: "ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells." In: DNA repair , Vol. 6, Issue 1, pp. 128-34, 2007 (PubMed)

Ramuirez, Stopper, Hock et al.: "Prevention of aneuploidy by S-adenosyl-methionine in human cells treated with sodium arsenite." In: Mutation research , Vol. 617, Issue 1-2, pp. 16-22, 2007 (PubMed)

Jacquemont, Taniguchi: "Proteasome function is required for DNA damage response and fanconi anemia pathway activation." In: Cancer research , Vol. 67, Issue 15, pp. 7395-405, 2007 (PubMed)

Sugimoto, Kitabayashi, Osano et al.: "Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1." In: Molecular biology of the cell , Vol. 19, Issue 3, pp. 1007-21, 2008 (PubMed)

Efeyan, Murga, Martinez-Pastor et al.: "Limited role of murine ATM in oncogene-induced senescence and p53-dependent tumor suppression." In: PloS one , Vol. 4, Issue 5, pp. e5475, 2009 (PubMed)

Boichuk, Hu, Hein et al.: "Multiple DNA Damage Signaling and Repair Pathways Deregulated by Simian Virus 40 Large T Antigen." In: Journal of virology , 2010 (PubMed)