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Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G) (AA 145-153), (C-Term) antibody

Details for Product No. ABIN954240, Supplier: Login to see
Antigen
  • POLR2G
  • RPB19
  • RPB7
  • hRPB19
  • hsRPB7
  • 2410046K11Rik
  • A230108L04Rik
  • C76415
  • RBP7
  • Rpo2-7l
  • Rpb7
  • Polr2g
  • wu:fc18d05
  • zgc:55297
Alternatives
anti-Human Polymerase (RNA) II (DNA Directed) Polypeptide G antibody for Flow Cytometry
Epitope
C-Term, AA 145-153
34
17
15
12
2
2
2
1
1
1
1
Reactivity
Human
65
4
3
3
1
1
1
1
Host
Rabbit
45
14
7
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
61
41
20
3
3
2
1
1
1
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 145-153 amino acids from the C-terminal region of Human POLR2G
Isotype Ig
Specificity This antibody recognizes Human POLR2G (C-term).
Purification Protein A column, followed by peptide affinity purification
Alternative Name POLR2G (POLR2G Antibody Abstract)
Background This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.Synonyms: DNA-directed RNA polymerase II subunit G, DNA-directed RNA polymerase II subunit RPB7, RNA polymerase II 19 kDa subunit, RNA polymerase II subunit B7, RPB19, RPB7
Molecular Weight 19294 Da
Gene ID 5436
NCBI Accession NP_002687
Pathways Regulatory RNA Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G) (AA 145-153), (C-Term) antibody (ABIN954240) Flow cytometric analysis of Jurkat cells using POLR2G Antibody (C-term) Cat.-No AP533...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G) (AA 145-153), (C-Term) antibody (ABIN954240) Western blot analysis of POLR2G Antibody (C-term) in Jurkat cell line lysates (35ug/l...
Background publications Cojocaru, Jeronimo, Forget et al.: "Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription." in: The Biochemical journal, Vol. 409, Issue 1, pp. 139-47, 2007 (PubMed).

Ujvári, Luse: "RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit." in: Nature structural & molecular biology, Vol. 13, Issue 1, pp. 49-54, 2006 (PubMed).

Meka, Werner, Cordell et al.: "Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II." in: Nucleic acids research, Vol. 33, Issue 19, pp. 6435-44, 2005 (PubMed).

Lehner, Semple, Brown et al.: "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region." in: Genomics, Vol. 83, Issue 1, pp. 153-67, 2003 (PubMed).

Zhou, Deng, Kashanchi et al.: "The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 22, pp. 12666-71, 2003 (PubMed).