PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 148-177)

Details for Product anti-PRKAA2 Antibody No. ABIN954298, Supplier: Log in to see
Antigen
  • ampk
  • ampk2
  • prkaa
  • prkaa1
  • PRKAA2
  • AMPK
  • AMPK2
  • AMPKa2
  • PRKAA
  • Ampk
  • Ampka2
  • 2310008I11Rik
  • A830082D05
  • AMPKalpha2
  • AMPKA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
  • prkaa2
  • PRKAA2
  • Prkaa2
Alternatives
anti-Human PRKAA2 antibody for ELISA
Epitope
AA 148-177, Middle Region, Thr172
42
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14
11
7
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4
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2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
238
129
100
14
10
6
6
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5
3
3
3
2
2
2
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2
Host
Rabbit
200
24
19
1
Clonality
Polyclonal
Conjugate
This PRKAA2 antibody is un-conjugated
12
11
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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104
61
39
31
22
18
16
6
4
3
2
1
1
1
1
Options
Supplier
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Immunogen KLH conjugated synthetic peptide between 148-177 amino acids from the Central region of human PRKAA2 (Thr172)
Isotype Ig
Specificity This antibody reacts to PRKAA2.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Alternative Name PRKAA2 (PRKAA2 Antibody Abstract)
Background The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-2 chain, AMPK2
Molecular Weight 62320 Da
Gene ID 5563
NCBI Accession NP_006243
Pathways AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 148-177) (ABIN954298) PRKAA2 (Thr172) Antibody (Center) flow cytometric analysis of K562 cells (right histo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 148-177) (ABIN954298) PRKAA2 (Thr172) antibody (Center) immunohistochemistry analysis in formalin fixed and...
Western Blotting (WB) image for anti-PRKAA2 antibody (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit) (AA 148-177) (ABIN954298) PRKAA2 (Thr172) Antibody western blot analysis in K562 cell line lysates (35µg/lane)....
Background publications Bungard, Fuerth, Zeng, Faubert, Maas, Viollet, Carling, Thompson, Jones, Berger: "Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation." in: Science (New York, N.Y.), Vol. 329, Issue 5996, pp. 1201-5, 2010 (PubMed).

Jablonski, McAteer, de Bakker, Franks, Pollin, Hanson, Saxena, Fowler, Shuldiner, Knowler, Altshuler, Florez: "Common variants in 40 genes assessed for diabetes incidence and response to metformin and lifestyle intervention in the diabetes prevention program." in: Diabetes, Vol. 59, Issue 10, pp. 2672-81, 2010 (PubMed).

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Ruaño, Thompson, Kane, Pullinger, Windemuth, Seip, Kocherla, Holford, Wu: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).