Smoothelin (SMTN) antibody

Details for Product No. ABIN954858
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019, smsmo
Reactivity
Chicken, Cat (Feline), Human, Monkey, Pig (Porcine), Rat (Rattus)
(9), (5), (3), (3), (3), (3), (1), (1)
Host
Mouse
(12)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(11), (10), (8), (3), (3), (3), (1), (1), (1)
Pubmed 7 references available
Quantity 1 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN954858
522.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Cytoskeletal extract of Chicken gizzard.
Clone R4A
Isotype IgG1
Specificity The antibody is very useful for characterization of atherosclerotic lesions. The antibody is useful tool to monitor SMC differentiation.
Purification Supernatant
Alternative Name Smoothelin
Background Smoothelin is a constituent of the smooth muscle cell cytoskeleton protein exclusively found in differentiated smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. To distinguish bladder muscularis mucosae (MM) from muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder carcinoma. Strong smoothelin expression is nearly exclusively observed in muscularis propria. Therefore, the staining pattern of MP (strongly positive) and MM (negative or weakly positive) makes this technique an attractive diagnostic tool for the sometimes difficult task of staging bladder urothelial carcinoma such as in transurethral resection specimens of urinary bladder tumors. Differentiating between smooth muscle tumors and other mesenchymal neoplasms of the GI tract can be challenging in small biopsies. Anti-smoothelin immunostaining can be helpful in differentiating benign (+) from malignant smooth muscle tumors (-), and other mimics(-).
Alternate names: SMSMO, SMTN
Gene ID 5395
NCBI Accession NP_000526
UniProt P53814
Research Area Organogenesis, Cytoskeleton
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or at -20°C for longer.
Expiry Date 12 months
Background publications van der Loop, Schaart, Timmer et al.: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).

Wehrens, Mies, Gimona et al.: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen et al.: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).

Christen, Bochaton-Piallat, Neuville et al.: "Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation." in: Circulation research, Vol. 85, Issue 1, pp. 99-107, 1999 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, 2000 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, 2000 (PubMed).

Deruiter, Rensen, Coolen et al.: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, 2001 (PubMed).

Validation Images
Did you look for something else?
back to top