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BCL2-Associated X Protein (BAX) antibody

Antigen

BCL2-Associated X Protein (BAX)
Synonyms BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA
Clonality Monoclonal (2D2)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
3 references available
Catalog no. ABIN95540
Quantity 6ml  (1 to 4 ug/ml)  (Variants)
Price 229.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Publications Alternatives Secondary Antibodies Variants back to top

Alternative name Bax
Immunogen BALB/C mice were injected with a synthetic peptide amino acids 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Format Supernatant (prediluted)
Isotype IgG1  (Matching secondary antibodies)
Clone 2D2
Specificity This antibody recognizes a 211 kD protein identified as bax protein. This antibody is highly specific to bax and does not cross react with bcl-2 alpha or bcl-X protein. This antibody does not cross react with mouse and rat.

Application Details
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Application Notes Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lymphoma. Cellular localization: Cell membrane, cytoplasm
Concentration 1 to 4 ug/ml
Buffer This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Apoptosis/Necrosis
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
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Publications Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, 1997 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).

Wolter, Hsu, Smith et al.: "Movement of Bax from the cytosol to mitochondria during apoptosis." in: The Journal of cell biology, Vol. 139, Issue 5, pp. 1281-92, 1997 (PubMed).

Alternatives
Application Details Publications Secondary Antibodies Variants back to top

Alternatives for antigen "BCL2-Associated X Protein (BAX)", type "Antibodies"
Hosts Mouse (77), Rabbit (68), Sheep (3)
Reactivities Human (130), Mouse (Murine) (69), Rat (Rattus) (65), Dog (Canine) (32), Cow (Bovine) (31), Pig (Porcine) (31), Sheep (Ovine) (30), Chicken (1), Horse (Equine) (1), Rabbit (1)
Applications Western Blotting (WB) (90), Immunofluorescence (IF) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunoprecipitation (IP) (37), Flow Cytometry (FACS) (36), ELISA (18), Immunohistochemistry (IHC) (12), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
Conjugates Biotin (8), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes N-Term (2), universal (1)