B-Cell CLL/lymphoma 2 (BCL2) antibody

Antigen: B-Cell CLL/lymphoma 2 (BCL2)

Synonyms: Bcl-2, bcl-2, BCL2

Clonality: Monoclonal (100-D5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN95566

Additional Information

Alternative name: Bcl-2

Immunogen: BALB/C mice were injected with synthetic bcl-2 peptide.

Format: Supernatant

Isotype: IgG1

Clone: 100-D5

Specificity: The bcl-2 product is considered to act as an inhibitor of apoptosis. bcl-2 is expression is inhibited in germinal centers where apoptosis forms part of B cell production pathway. Reactive follicle show no staining for bcl-2, where as the cells in neoplastic follicles exhibit membrane staining.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane Other Applications: Immunoprecipitation (1:50), Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

LeBrun, Kamel, Cleary et al.: "Follicular lymphomas of the gastrointestinal tract. Pathologic features in 31 cases and bcl-2 oncogenic protein expression." in: The American journal of pathology, Vol. 140, Issue 6, pp. 1327-35, 1992 (PubMed).

Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).