B-Cell CLL/lymphoma 2 (BCL2) antibody
| Antigen |
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| Synonyms |
Bcl-2, bcl-2, BCL2 |
| Clonality |
Monoclonal (124) |
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Host
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Reactivity
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Human (223), Mouse (Murine) (81), Rat (Rattus) (64), Dog (Canine) (54), Cow (Bovine) (44), Pig (Porcine) (42), Horse (Equine) (41), Chicken (19), Guinea Pig (18), Sheep (Ovine) (5), Rabbit (3), Cat (Feline) (1)
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Conjugate
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Biotin (10), FITC (9), PE (7), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RPE (1)
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (161), Immunofluorescence (IF) (88), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (86), ELISA (79), Immunohistochemistry (IHC) (67), Immunoprecipitation (IP) (56), Flow Cytometry (FACS) (37), Immunocytochemistry (ICC) (12), Immunohistochemistry (Fixed) (IHC (fx)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Dot Blot (Dot) (6), Immunoelectron Microscopy (IEM) (2), Cellular Assay (CA) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN95567 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
442.20 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 3 to 4 Business Days |
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Alternative name
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Bcl-2
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Immunogen
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Recombinant bcl-2 peptide sequence comprising of 51-54 amino acids of bcl-2 oncoprotein.
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Format
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Supernatant
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Isotype
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IgG1 kappa
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Clone
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124
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Specificity
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This antibody is specific to bcl-2 oncoprotein, encoded by a gene involved in the 14, 18 chromosomal translocation. In lymphoid tissues, this antibody stains B- lymphocytes as well as T cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Cell membrane Other Applications: Western Blotting (1:100-750)
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Concentration
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100ug/ml approximately
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Purification
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Supernatant
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Buffer
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o C. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).
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Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
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Hosts
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Rabbit (166), Mouse (79), Goat (7), Sheep (3), Pig (1)
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Reactivities
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Human (223), Mouse (Murine) (81), Rat (Rattus) (64), Dog (Canine) (54), Cow (Bovine) (44), Pig (Porcine) (42), Horse (Equine) (41), Chicken (19), Guinea Pig (18), Sheep (Ovine) (5), Rabbit (3), Cat (Feline) (1)
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Applications
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Western Blotting (WB) (161), Immunofluorescence (IF) (88), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (86), ELISA (79), Immunohistochemistry (IHC) (67), Immunoprecipitation (IP) (56), Flow Cytometry (FACS) (37), Immunocytochemistry (ICC) (12), Immunohistochemistry (Fixed) (IHC (fx)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Dot Blot (Dot) (6), Immunoelectron Microscopy (IEM) (2), Cellular Assay (CA) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
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Conjugates
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Biotin (10), FITC (9), PE (7), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RPE (1)
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Epitopes
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AA 41-54 (16), N-Term (16), pSer70 (13), pThr56 (12), pThr129 (5), AA 61-76 (4), Internal Region (4), BH3 Domain (3), AA 51-54 (2), AA 67-86 (2), C-Term (2), Center (2), AA 1-195 (1), AA 21-34 (1), Middle Region (1), Ser70 (1), Thr56 (1), pSer592 (1), pSer87 (1), pSer99 (1), pThr69 (1)
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