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B-Cell CLL/lymphoma 2 (BCL2) antibody

Antigen

B-Cell CLL/lymphoma 2 (BCL2)

Synonyms Bcl-2, bcl-2, BCL2
Clonality Monoclonal (124)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1 reference available
Catalog no. ABIN95567
Quantity 1ml  (100ug/ml approximately)  (Variants)
Price 442.20 $   Plus shipping costs $45.00
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Availability Will be delivered in 3 to 4 Business Days

Additional Information

Alternative name Bcl-2
Immunogen Recombinant bcl-2 peptide sequence comprising of 51-54 amino acids of bcl-2 oncoprotein.
Format Supernatant
Isotype IgG1 kappa
Clone 124
Specificity This antibody is specific to bcl-2 oncoprotein, encoded by a gene involved in the 14, 18 chromosomal translocation. In lymphoid tissues, this antibody stains B- lymphocytes as well as T cells.

Application Details

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Cell membrane Other Applications: Western Blotting (1:100-750)
Concentration 100ug/ml approximately
Purification Supernatant
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o C. Do not use it beyond the expiration date stated on the label.
Research Area Cancer
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Alternatives

Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
Hosts Rabbit (166), Mouse (79), Goat (7), Sheep (3), Pig (1)
Reactivities Human (223), Mouse (Murine) (81), Rat (Rattus) (64), Dog (Canine) (54), Cow (Bovine) (44), Pig (Porcine) (42), Horse (Equine) (41), Chicken (19), Guinea Pig (18), Sheep (Ovine) (5), Rabbit (3), Cat (Feline) (1)
Applications Western Blotting (WB) (161), Immunofluorescence (IF) (88), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (86), ELISA (79), Immunohistochemistry (IHC) (67), Immunoprecipitation (IP) (56), Flow Cytometry (FACS) (37), Immunocytochemistry (ICC) (12), Immunohistochemistry (Fixed) (IHC (fx)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Dot Blot (Dot) (6), Immunoelectron Microscopy (IEM) (2), Cellular Assay (CA) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
Conjugates Biotin (10), FITC (9), PE (7), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RPE (1)
Epitopes AA 41-54 (16), N-Term (16), pSer70 (13), pThr56 (12), pThr129 (5), AA 61-76 (4), Internal Region (4), BH3 Domain (3), AA 51-54 (2), AA 67-86 (2), C-Term (2), Center (2), AA 1-195 (1), AA 21-34 (1), Middle Region (1), Ser70 (1), Thr56 (1), pSer592 (1), pSer87 (1), pSer99 (1), pThr69 (1)