B-Cell CLL/lymphoma 2 (BCL2) antibody
| Antigen |
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| Synonyms |
Bcl-2, bcl-2, BCL2 |
| Clonality |
Monoclonal (124) |
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Host
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Reactivity
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Human (284), Mouse (Murine) (126), Rat (Rattus) (113), Dog (Canine) (81), Cow (Bovine) (56), Pig (Porcine) (55), Horse (Equine) (54), Chicken (19), Guinea Pig (18), Sheep (Ovine) (18), Cat (Feline) (3), Rabbit (3), Gerbil (1)
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Conjugate
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Biotin (13), FITC (13), PE (10), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), Gold (5), HRP (5), PE,Cy3 (5), PE,Cy5 (5), PE,Cy5.5 (5), PE,Cy7 (5), RPE (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (195), Immunofluorescence (IF) (120), ELISA (106), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (98), Immunohistochemistry (IHC) (65), Immunoprecipitation (IP) (65), Flow Cytometry (FACS) (40), Immunocytochemistry (ICC) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (19), Immunohistochemistry (Fixed) (IHC (fx)) (12), Dot Blot (Dot) (6), Immunoelectron Microscopy (IEM) (5), Dot Blot (DB) (2), Cellular Assay (CA) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN95568 |
| Quantity |
6ml (1 to 4 ug/ml) (Variants) |
| Price |
227.70 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 3 to 4 Business Days |
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Alternative name
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Bcl-2
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Immunogen
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BALB/C mice were immunized with synthetic peptide sequence comprising amino acids 51-54 of bcl-2 protein.
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Format
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Supernatant (prediluted)
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Isotype
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IgG1 kappa
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Clone
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124
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Specificity
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This antibody reacts with a 25 kD bcl protein, which lies within the cell rather than on the cell surface. It stains neoplastic cells of follicular lymphoma, hairy cell leukemia, high grade B and T cell lymphomas, lymphoblastic lymphomas and anaplastic large cell lymphoma.
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Application Notes
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Overfixation in formalin should be avoided, as it can give inconsistent results. Suitable for formalin/paraffin tissues. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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1 to 4 ug/ml
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Purification
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Supernatant (prediluted)
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).
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Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
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Hosts
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Rabbit (233), Mouse (86), Goat (7), Sheep (3), Pig (1)
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Reactivities
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Human (284), Mouse (Murine) (126), Rat (Rattus) (113), Dog (Canine) (81), Cow (Bovine) (56), Pig (Porcine) (55), Horse (Equine) (54), Chicken (19), Guinea Pig (18), Sheep (Ovine) (18), Cat (Feline) (3), Rabbit (3), Gerbil (1)
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Applications
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Western Blotting (WB) (195), Immunofluorescence (IF) (120), ELISA (106), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (98), Immunohistochemistry (IHC) (65), Immunoprecipitation (IP) (65), Flow Cytometry (FACS) (40), Immunocytochemistry (ICC) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (19), Immunohistochemistry (Fixed) (IHC (fx)) (12), Dot Blot (Dot) (6), Immunoelectron Microscopy (IEM) (5), Dot Blot (DB) (2), Cellular Assay (CA) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
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Conjugates
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Biotin (13), FITC (13), PE (10), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), Gold (5), HRP (5), PE,Cy3 (5), PE,Cy5 (5), PE,Cy5.5 (5), PE,Cy7 (5), RPE (1)
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Epitopes
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pSer70 (27), pThr56 (27), N-Term (23), C-Term (20), AA 41-54 (19), pThr129 (19), pSer70,Internal Region (6), AA 61-76 (4), BH3 Domain (3), Internal Region (3), AA 51-54 (2), AA 67-86 (2), Center (2), AA 1-195 (1), AA 21-34 (1), Internal Region,AA 143-171 (1), Internal Region,AA 47-74 (1), Internal Region,AA 85-114 (1), Internal Region,AA 87-116 (1), Middle Region (1), N-Term,AA 38-64 (1), Ser70 (1), Thr56 (1), pSer87 (1), pThr69 (1)
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