B-Cell CLL/lymphoma 6 (BCL6) antibody

Antigen: B-Cell CLL/lymphoma 6 (BCL6)

Synonyms: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27, bcl5, laz3, bcl6a, znf51, zbtb27, MGC132260, BCL6, DKFZp468I1913

Clonality: Monoclonal (PG-B6P)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN95578

Additional Information

Alternative name: Bcl-6

Immunogen: Recombinant protein corresponding to amino acids 3-484 of the human bcl-6 protein.

Format: Supernatant (prediluted)

Isotype: IgG1  (Matching secondary antibodies)

Clone: PG-B6P

Specificity: This antibody reacts with bcl-6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitts lymphomas and in nodular, lymphocyte predominant Hodgkins disease. bcl-6 is a proto-oncogene that encodes Kruppel-type Zinc-finger protein of 95 kD and shares homology with transcription factor. Bcl-6 is mainly expressed in normal germinal center B cells and related lymphomas. This antibody cross reacts with human, cow, rabbit, rat, sheep and pig.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking using 1mM EDTA (pH 8.0) is recommended for formalin fixed paraffin tissues sections. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Pescarmona, Lo Coco, Pacchiarotti et al.: "Analysis of the BCL-6 gene configuration in diffuse B-cell non-Hodgkin's lymphomas and Hodgkin's disease." in: The Journal of pathology, Vol. 177, Issue 1, pp. 21-5, 1995 (PubMed).

Otsuki, Yano, Clark et al.: "Analysis of LAZ3 (BCL-6) status in B-cell non-Hodgkin's lymphomas: results of rearrangement and gene expression studies and a mutational analysis of coding region sequences." in: Blood, Vol. 85, Issue 10, pp. 2877-84, 1995 (PubMed).

Flenghi, Bigerna, Fizzotti et al.: "Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region." in: The American journal of pathology, Vol. 148, Issue 5, pp. 1543-55, 1996 (PubMed).