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Bcl-X antibody
| Antigen | Bcl-X |
| Clonality | Monoclonal (2H12) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
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3 references available |
| Catalog no. | ABIN95584 |
| Quantity | 1ml (100ug/ml approximately) (Variants) |
| Price | 518.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Immunogen | BALB/C mice were injected with a synthetic peptide of amino acid 3-14 (Cys-QSNRELVVDFLS) of human bcl-X protein. |
| Format | Supernatant |
| Isotype | IgG2a (Matching secondary antibodies) |
| Clone | 2H12 |
| Specificity | This antibody recognizes a 27 kD protein identified as bcl-XL protein. This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein. This antibody cross reacts with human, mouse, rat and pig. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Hodgkin |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Cancer |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
| Publications |
Hsu, Wolter, Youle: "Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3668-72, 1997 (PubMed).
Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed). Wolter, Hsu, Smith et al.: "Movement of Bax from the cytosol to mitochondria during apoptosis." in: The Journal of cell biology, Vol. 139, Issue 5, pp. 1281-92, 1997 (PubMed). |
Alternatives
Alternatives for antigen "Bcl-X", type "Antibodies"
| Hosts | Rabbit (6), Mouse (1) |
| Reactivities | Human (6), Rat (Rattus) (3), Mouse (Murine) (2) |
| Applications | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Western Blotting (WB) (4), Immunohistochemistry (IHC) (3), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1) |
