Mucin 16, Cell Surface Associated (MUC16) antibody

Antigen: Mucin 16, Cell Surface Associated (MUC16)

Synonyms: CA125, FLJ14303, Ca125, Gm1480, 1110008I14Rik, RGD1311421, RGD1308422, MUC16

Clonality: Monoclonal (OV185:1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN95819

Additional Information

Alternative name: CA 125

Immunogen: Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: OV185:1

Specificity: This antibody reacts specifically with CA125 ovarian cancer antigen. Several studies have shown that CA125 is useful tumor marker for ovarian epithelial malignancies. However, CA125 has been described in other neoplasms such as seminal vesicle and anaplastic lymphomas.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Ovarian carcinoma. Cellular localization: Extracellular membrane

Concentration: 100ug/ml approximately

Buffer: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Ohmori, Okada, Tabei et al.: "CA125-producing adenocarcinoma of the seminal vesicle." in: Pathology international, Vol. 44, Issue 4, pp. 333-7, 1994 (PubMed).

Alagoz, Buller, Berman et al.: "What is a normal CA125 level?" in: Gynecologic oncology, Vol. 53, Issue 1, pp. 93-7, 1994 (PubMed).