Calbindin 1, 28kDa (CALB1) antibody

Antigen: Calbindin 1, 28kDa (CALB1)

Synonyms: CALB, CB, Calb, Calb-1, Brain-2, DCBP, Dcp, cbn, cbp53E, DmelCG6702, CG6702, CaBP28K, MGC93326, CALB1, MGC138959, calb1, Calbindin, DKFZp459E0414

Clonality: Monoclonal (CB-955)

Host: Mouse

Reactivity: Human, Monkey, Rabbit, Rat (Rattus), Mouse (Murine), Chicken, Hamster, Sheep (Ovine), Guinea Pig, Fish

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN95842

Additional Information

Alternative name: Calbindin

Immunogen: Purified Calbindin-D from chicken gut was used as the immunogen.

Format: Ascites

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: CB-955

Specificity: This antibody is specific to a protein of 28 kD. Calbindin-D occurs only in a subset of neurons and in a few other tissues. This antibody reacts specifically with Calbindin-D in brain and kidney tissue from human, monkey, rabbit, rat, mouse, chicken, hamster, sheep, guinea pig and fish.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15 to 1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Kidney. Cellular localization: Cytoplasmic, Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Chilosi, Doglioni, Pelosio et al.: "Detection of calbindin-D immunoreactivity in human follicular dendritic cells." in: The Journal of pathology, Vol. 166, Issue 2, pp. 121-7, 1992 (PubMed).

Katsetos, Jami, Krishna et al.: "Novel immunohistochemical localization of 28,000 molecular-weight (Mr) calcium binding protein (calbindin-D28k) in enterochromaffin cells of the human appendix and neuroendocrine tumors (carcinoids and small-cell carcinomas) of the midgut and foregut." in: Archives of pathology & laboratory medicine, Vol. 118, Issue 6, pp. 633-9, 1994 (PubMed).