EHF antibody (AA 121-170)

Catalog No. ABIN6748571

Product Details anti-EHF Antibody

Target: EHF (Ets Homologous Factor (EHF))

Binding Specificity: AA 121-170

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Mouse EHF

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Dog, Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of mouse Ehf (O70273, NP_031940). Percent identity by BLAST analysis: Mouse, Rat (100%), Elephant (92%), Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Platypus (85%), Human (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. Antibody does not work for IHC with mouse samples.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EHF

Target: EHF (Ets Homologous Factor (EHF))

Alternative Name: ESE3 / EHF (EHF Products)

Synonyms: zgc:136967 antibody, ESE3 antibody, ESE3B antibody, ESEJ antibody, 9030625L19Rik antibody, AU019492 antibody, ets homologous factor antibody, ETS homologous factor antibody, ehf antibody, EHF antibody, Ehf antibody

Background: Name/Gene ID: EHF

Synonyms: EHF, ESE3, ESE3B, ESE-3, ESE3 transcription factor, Ets homologous factor, ESEJ, HEHF

Gene ID: 26298

NCBI Accession: NP_031940

UniProt: Q9NZC4